mmgA [2019-06-26 11:22:27]

degradative acetoacetyl-CoA thiolase

locus: BSU24170

pI: 5.90

mw: 41.02 kDa

protein length: 393 aa

gene length: 1182 bp

function: mother cell metabolism, leucine utilization

product: degradative acetoacetyl-CoA thiolase

essential: no

ec: 2.3.1.9

synonyms: yqiL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 2,512,861 2,514,042

The protein

Catalyzed reaction/ biological activity

• 2 acetyl-CoA = CoA + acetoacetyl-CoA [Pubmed|19935659]

Protein family

• thiolase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|YhfS], [protein|07498A0A3CB598A6E388540124A4F56E781C86FA|FadA]

Structure

• [PDB|3SS6] (the protein of ''B. anthracis'', 44% identity, 73% similarity)

Expression and Regulation

Operons

• genes: [gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]-[gene|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB]-[gene|F25CDDEBAFC72036664323619F5197D45E86BE9A|mmgC]-[gene|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|mmgD]-[gene|602EAB591A550F68C14F35F6E5F0A91C3B997343|mmgE]-[gene|2F4A025B8E3F14764D24CD7E33F7608C2EB1C42C|mmgF]
  description: [Pubmed|8759838]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,8759838], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8759838], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|15699190,8759838]
  • strongly expressed during oligotrophic growth [pubmed|30792386]

Biological materials

Mutant

• BKE24170 ([gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG
• BKK24170 ([gene|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|mmgA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG

References

• 18246303,8759838,15699190,19935659,23840410