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mmgA [2019-06-26 11:22:27]
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mmgA [2019-06-26 11:22:27]

degradative acetoacetyl-CoA thiolase
Locus
BSU24170
Isoelectric point
5.90
Molecular weight
41.02 kDa
Protein length
393 aa Sequence Blast
Gene length
1182 bp Sequence Blast
Function
mother cell metabolism, leucine utilization
Product
degradative acetoacetyl-CoA thiolase
Essential
no
E.C.
2.3.1.9
Synonyms
yqiL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

    • Gene

    Coordinates
    2,512,861 2,514,042

    The protein

    Catalyzed reaction/ biological activity

  • 2 acetyl-CoA = CoA + acetoacetyl-CoA PubMed

    • Protein family

  • thiolase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • YhfS, FadA

    • Structure

  • 3SS6 (the protein of B. anthracis, 44% identity, 73% similarity)

    • Expression and Regulation

    Operons

    Genes
    mmgA-mmgB-mmgC-mmgD-mmgE-mmgF
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed
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    Biological materials

    Mutant

  • BKE24170 (mmgA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG
  • BKK24170 (mmgA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTTCACCTCATTCAG, downstream forward: _UP4_TAAAACATGATGAAAAAGGG

    • References

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